Screen with invstigation results

Confocal Microscopy can be used for the generation of confocal images of fixed samples and the investigation of living cells with advanced fluorescence techniques.


Confocal images

  • Pre-experimental consulting and hands-on training
  • 2-dimensional confocal images
    • Multi-channel images with up to 4 fluorophores
    • Exact overlay with differential interference contrast (DIC) images
    • Fluorescence intensity profiles
    • Colocalization analysis
  • 3-dimensional confocal images
    • Preparation of image stacks („z-stacks“)
    • Reconstruction of 3-dimensional objects

Advanced fluorescence techniques

  • Pre-experimental consulting and hands-on training
  • FRAP, Fluorescence Recovery After Photobleaching,
    and FLIP, Fluorescence Loss In Photobleaching, to determine the mobility of fluorescently labelled molecules in living cells
  • FRET, Förster Resonance Energy Transfer, to detect interaction of fluorescently labeled molecules
  • Use of photoconvertible and photoactivatable fluorescent proteins to determine protein dynamics in living cells
  • Live-cell imaging with temperature- and CO2-control