Service

  Screen with invstigation results

Confocal Microscopy can be used for the generation of confocal images of fixed samples and the investigation of living cells with advanced fluorescence techniques.

 

Confocal images

  • Pre-experimental consulting and hands-on training
  • 2-dimensional confocal images
    • Multi-channel images with up to 4 fluorophores
    • Exact overlay with differential interference contrast (DIC) images
    • Fluorescence intensity profiles
    • Colocalization analysis
  • 3-dimensional confocal images
    • Preparation of image stacks („z-stacks“)
    • Reconstruction of 3-dimensional objects

Advanced fluorescence techniques

  • Pre-experimental consulting and hands-on training
  • FRAP, Fluorescence Recovery After Photobleaching,
    and FLIP, Fluorescence Loss In Photobleaching, to determine the mobility of fluorescently labelled molecules in living cells
  • FRET, Förster Resonance Energy Transfer, to detect interaction of fluorescently labeled molecules
  • Use of photoconvertible and photoactivatable fluorescent proteins to determine protein dynamics in living cells
  • Live-cell imaging with temperature- and CO2-control