Equipment/Instruments

  Genomics Facility Copyright: Denecke

The facility’s confined laboratory is adapted to also working with nucleic acids available either in limited quantity and / or degraded quality.

Procedures and infrastructure are in place to prevent any risk of contamination by nucleic acids of exogenous origin.

Microarrays equipment

The Affymetrix Array System is composed of 2 wash stations (GeneChip Fluidics Station 450), a hybridisation oven (GeneChip Hybridisation oven 645), a scanner (GeneChip Scanner 3000 7G) and a computer workstation. A Glass Array 428 Scanner (Affymetrix) is also available at the facility.

NGS equipment

The NextSeq500 from Illumina is capable of performing runs from 130 to 400 reads/run (single-end or paired-end sequencing). Its high data quality provides a reliable source for whole-genome, transcriptome, and other sequencing projects.

Bioinformatics

  • RECT Rack Server RS-8685MR8 (Coreto)
  • RECT Storage ST-38R24-D (Coreto)
  • RECT Rack Server RS-8634R8 (Coreto)

DNA & RNA isolation

  • Maxwell RSC (Promega)

Quantification of DNA & RNA

  • Nanodrop ND-1000 (Thermo Scientific)
  • Quantus (Promega)

Fragmentation of DNA & RNA

  • Covaris M220

Quality Control of DNA, RNA & Protein

  • Tape Station 4200 (Agilent)
  • Bioanalyser (Agilent)

PCR Amplification

  • 7300 Real Time PCR System (Applied Biosystems)
  • Gene Amp PCR 9700 (Applied Biosystems)
  • PTC-200 (Pelletier Thermal Cycler, MJ Research)