Two-Photon Imaging Facility
The Two-Photon-Imaging Facility of the IZKF Aachen, located in the Institute for Laboratory Animal Science, includes two multi-photon microscope systems.
The imaging systems TriMScope I (LaVision BioTec) and Fluoview FV1000MPE (Olympus) enable in vitro, ex vivo, in situ and in vivo investigations.
Beside microscopic experiments two in vivo optical imaging systems, integrated in the core facility, allow the non-invasive imaging of fluorescence or bioluminescence signals in small animals (Perkin Elmer IVIS Lumina XR and Berthold NightOWL).
Two-photon laser scanning microscopy (TPLSM)
TPLSM is based on the principle of two-photon excitation by a near infrared (NIR) laser and enables fluorescence imaging of optical slides at subcellular resolution. NIR light penetrates deeper into scattering tissue and thus allows imaging in intact samples at higher depth. Image stacks over a definite depth range can be used for spatial reconstruction to visualize fluorescent structures in 3D. These features makes TPLSM advantageous over other microscopic techniques for visualization of structures located deeper in scattering or vulnerable tissues.
In-vivo optical imaging
The in-vivo optical imaging systems enable non-invasive whole-body imaging of fluorescence or bioluminescence signals in superposition with photographs or radiographs. The anatomical localization of fluorescent dyes or bioluminescent reporters can be imaged non-invasively in living small animals over extended time periods in the same animal. In addition, investigations of cell cultures and ex vivo samples are possible.