Service

 

Confocal Microscopy can be used for the generation of confocal images of fixed samples and the investigation of living cells with advanced fluorescence techniques.

 

Confocal images:

• Pre-experimental consulting and hands-on training
• 2-dimensional confocal images

- Multi-channel images with up to 4 fluorophores

- Exact overlay with differential interference contrast (DIC) images

- Fluorescence intensity profiles

- Colocalization analysis

• 3-dimensional confocal images

- Preparation of image stacks („z-stacks“)

- Reconstruction of 3-dimensional objects

Advanced fluorescence techniques:

• Pre-experimental consulting and hands-on training
• FRAP (fluorescence recovery after photobleaching) and
• FLIP (fluorescence loss in photobleaching) to determine the mobility of fluorescently labelled molecules in living cells
• FRET (Förster resonance energy transfer) to detect interaction of fluorescently labeled molecules
• Use of photoconvertible and photoactivatable fluorescent proteins to determine protein dynamics in living cells
• Spectral imaging and linear unmixing for the separation of more than 4 fluorophores
• Single-molecule RNA fluorescence in situ hybridization (smRNA-FISH)
• Super-resolution microscopy based on single molecule localization such as dSTORM
• Live-cell imaging with temperature- and CO₂-control