Service

  © Proteomics Facility  

What services do we offer?

 

• Pre-experimental consulting
  • Sample generation, experimental conditions, biochemical prerequisites
     
• Sample preparation
  • Lysis of cells, tissues, organs
  • 1D SDS-PAGE
  • Proteolytic digestion of the sample using specific proteases (Trypsin, LysC, GluC, etc.)
  • Peptide fractionation (reversed phase, ion exchange, and affinity chromatography), enrichment of phosphopeptides (pSer, pThr and pTyr) using TiO₂/Ti-IMAC or antibodies
  • Mass spectrometry analysis (qualitative and quantitative – SILAC, iTRAQ, `dimethyl, label free) from single proteins up to highly complex protein/peptide mixtures (cell lysates, immunoprecipitated complexes), analysis of post-translationally modified peptides
  •  Data analysis (peptide/protein identification and quantification)
     
• Data interpretation and advice on follow-up experiments