3X internal non-direct scanned PMT detectors (d=25mm). Bandwidth selection of emitted light by filters in motorized filter wheel is in front of each PMT (confocal detectors).
4X ultra-sensitive external non-descanned PMT detectors (d=32mm) for direct detection of emitted light. Bandwidth selection by spectral filters and corresponding dichroic mirrors (in a filter cube) in front of each PMT.

Windows 10 Prof. 64bit system; Intel Xeon CPU E5-1620-0 @ 3,60GHz/3,60GHz, 8 GB RAM with FluoView interface board 30 inch TFT- monitor.

Classic HBO fluorescent light source including light guide, three fluorescent filter cubes (DAPI/GFP/RHOD).
CCD camera Olympus XM10 (12 bit monochrome camera with Cell-P acquisition software.
Laser safety glasses and IR-viewer.

Microscope table:

Newport anti-vibration table (active dampening)

Laser:

Spectra physics IR Laser Insight X3 DUAL (700-1300 nm + 1045 nm), dual beamrouting with VBE.
Laser diodes with AOTF 488 nm, 552 nm, 638 nm

Scanner:

TCS SP8 Scan head (resonant/non-resonant)

Microscope stand:

Leica DM6 CFS (motorized upright microscope)

Detection:

Confocal detectors:

2x HyD SP gated, 1x HyD S SP gated (FLIM capable)

4TUNE DIVE (4-channel):

4X non-descanned 4Tune HyD RLD detectors (FLIM capable) with free tunable detection range

SP8 FALCON (FAst Lifetime CONtrast) with LAS X Phasor FLIM Online

Transmission detectors: 2 PMT with filter block

Microscope stage:

Scientifica MMBP, Okolab laser security box

Image acquisition computer:

Expert CUDA Workstation, RAID

High Power HPZ6G4 Workstation Windows 10 Professional (64 bit)
Intel 6134 XEON3.2 8C, 192 GByte RAM, NVIDIA Quadro GP100 16GB

Promise Pegasus3 RAID System (16 TB)
2 x 31,5 inch displays

Others:

EL 6000 (LQ-HXP 120 LEJ) fluorescent light source,
three fluorescent filter cubes (DAPI/GFP/RHOD).
Detection-Software LAS X

Objectives:

FLUOTAR L 16x/0.6 IMM CORR VISIR
HC PL APO 63x/1.2 W CORR CS2
HC IRAPO L 25x / 1.00 W motCORR

16x
63x
25x

NA 0.60
NA 1.20
NA 1.00

WD2.5
WD0.3
WD2.6