Proteomics Facility
Proteomics has become a standard analytical and extremely powerful research technology in essentially all research conducted within the medical faculty.
Proteomics is the analysis of all proteins as well as their post-translational modifications (PTMs) in cells, tissues, body fluids such as serum/plasma or entire organisms expressed by the respective genome at a given time point. This can be extended to in depth quantitative analysis of changes in protein and PTM levels in response to defined stimuli or disease for example oncogenic states. These goals are usually achieved by a combination of sample separation techniques such as chromatography approaches and ultra high resolution mass spectrometry (nanoLC-MS/MS).
The proteomics facility can aid in answering medically relevant questions, such as differences in tissue proteomes for example liver or kidney samples from transgenic mice vs wt littermates or changes in the phosphorylation pattern of the cellular proteome inferred by oncogenic signaling processes by using quantitative nanoLC-MS/MS.
The proteomics facility provides advice and support for the generation of such experimental setups and workflows based on the individual scientific question of the respective research group. This includes advice and suggestions for the process of appropriate sample generation including proper controls. The facility also offers experimental support in protein enrichment strategies such as co-IPs and affinity purification as well as PTM-specific protein and peptide enrichment strategies such as phosphopeptide analysis.